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polyclonal antibody against tslp  (R&D Systems)


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    Structured Review

    R&D Systems polyclonal antibody against tslp
    ( A ) Cytokine mRNA levels in BxPC-3, PT-45 and Capan-2 cells were assessed by real time RT-PCR and normalized to β-actin mRNA levels. Values are means (±SD) normalized gene expression. ( B ) Concentration of <t>TSLP</t> in CM derived from BxPC-3, PT-45, and Capan-2 cells measured by ELISA. The mean level (±SD) of cytokine detected in triplicate in CM samples is indicated.
    Polyclonal Antibody Against Tslp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody against tslp/product/R&D Systems
    Average 92 stars, based on 10 article reviews
    polyclonal antibody against tslp - by Bioz Stars, 2026-04
    92/100 stars

    Images

    1) Product Images from "Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance"

    Article Title: Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance

    Journal: Oncotarget

    doi: 10.18632/oncotarget.25997

    ( A ) Cytokine mRNA levels in BxPC-3, PT-45 and Capan-2 cells were assessed by real time RT-PCR and normalized to β-actin mRNA levels. Values are means (±SD) normalized gene expression. ( B ) Concentration of TSLP in CM derived from BxPC-3, PT-45, and Capan-2 cells measured by ELISA. The mean level (±SD) of cytokine detected in triplicate in CM samples is indicated.
    Figure Legend Snippet: ( A ) Cytokine mRNA levels in BxPC-3, PT-45 and Capan-2 cells were assessed by real time RT-PCR and normalized to β-actin mRNA levels. Values are means (±SD) normalized gene expression. ( B ) Concentration of TSLP in CM derived from BxPC-3, PT-45, and Capan-2 cells measured by ELISA. The mean level (±SD) of cytokine detected in triplicate in CM samples is indicated.

    Techniques Used: Quantitative RT-PCR, Gene Expression, Concentration Assay, Derivative Assay, Enzyme-linked Immunosorbent Assay

    Incidence of OX40L expression on normal HLA-DR + DCs obtained ( A ) in the absence (control), and in the presence of 20% BxPC-3 or Capan-2 cell-CM. Values are means ± SE. P -values obtained by One Way ANOVA followed by Bonferroni t -Test, or ( B ) in the presence of BxPC-3 cell-CM, pre-treated with a neutralizing anti-TSLP polyclonal antibody. Values are means ± SE. P -values obtained by the Paired t -Test.
    Figure Legend Snippet: Incidence of OX40L expression on normal HLA-DR + DCs obtained ( A ) in the absence (control), and in the presence of 20% BxPC-3 or Capan-2 cell-CM. Values are means ± SE. P -values obtained by One Way ANOVA followed by Bonferroni t -Test, or ( B ) in the presence of BxPC-3 cell-CM, pre-treated with a neutralizing anti-TSLP polyclonal antibody. Values are means ± SE. P -values obtained by the Paired t -Test.

    Techniques Used: Expressing, Control

    Basal circulating TSLP levels in PDAC patients ( n = 82) and healthy donors ( n = 41) as determined by ELISA. P -values obtained by the Mann-Whitney Rank Sum Test. Median, 10th, 25th, 75th, and 90th percentiles are presented as vertical boxes with error bars. Dots indicate outliers.
    Figure Legend Snippet: Basal circulating TSLP levels in PDAC patients ( n = 82) and healthy donors ( n = 41) as determined by ELISA. P -values obtained by the Mann-Whitney Rank Sum Test. Median, 10th, 25th, 75th, and 90th percentiles are presented as vertical boxes with error bars. Dots indicate outliers.

    Techniques Used: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Plasma levels of  TSLP  pre- and post-treatment
    Figure Legend Snippet: Plasma levels of TSLP pre- and post-treatment

    Techniques Used: Clinical Proteomics



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    Intestinal levels of IL-25 ( a ), IL-33 ( b ), and thymic stromal lymphopoietin <t>(TSLP)</t> ( c ) were evaluated by quantitative real-time PCR and immunohistochemistry (IHC). Left: values are presented as the mean ± SD of at least eight individuals in each group. *, **, P < 0.05 and 0.01 vs. the control or phototherapy group, respectively (Student’s t test). The pictures at ×400 magnification are representative of at least six individuals in each group. Scale bar: 20 μm.
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    R&D Systems polyclonal antibody against tslp
    ( A ) Cytokine mRNA levels in BxPC-3, PT-45 and Capan-2 cells were assessed by real time RT-PCR and normalized to β-actin mRNA levels. Values are means (±SD) normalized gene expression. ( B ) Concentration of <t>TSLP</t> in CM derived from BxPC-3, PT-45, and Capan-2 cells measured by ELISA. The mean level (±SD) of cytokine detected in triplicate in CM samples is indicated.
    Polyclonal Antibody Against Tslp, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody against tslp/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    polyclonal antibody against tslp - by Bioz Stars, 2026-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    Intestinal levels of IL-25 ( a ), IL-33 ( b ), and thymic stromal lymphopoietin (TSLP) ( c ) were evaluated by quantitative real-time PCR and immunohistochemistry (IHC). Left: values are presented as the mean ± SD of at least eight individuals in each group. *, **, P < 0.05 and 0.01 vs. the control or phototherapy group, respectively (Student’s t test). The pictures at ×400 magnification are representative of at least six individuals in each group. Scale bar: 20 μm.

    Journal: NPJ Biofilms and Microbiomes

    Article Title: Daily full spectrum light exposure prevents food allergy-like allergic diarrhea by modulating vitamin D 3 and microbiota composition

    doi: 10.1038/s41522-021-00213-8

    Figure Lengend Snippet: Intestinal levels of IL-25 ( a ), IL-33 ( b ), and thymic stromal lymphopoietin (TSLP) ( c ) were evaluated by quantitative real-time PCR and immunohistochemistry (IHC). Left: values are presented as the mean ± SD of at least eight individuals in each group. *, **, P < 0.05 and 0.01 vs. the control or phototherapy group, respectively (Student’s t test). The pictures at ×400 magnification are representative of at least six individuals in each group. Scale bar: 20 μm.

    Article Snippet: After peroxidase blocking, antigen retrieval and blocking, the slides were incubated with rat monoclonal antibody against Mcpt-1 (200× dilution; Thermo Fisher Scientific Inc.), mouse monoclonal antibody against IL-25 (500× dilution; Thermo Fisher Scientific Inc.), mouse monoclonal antibody against IL-33 (200× dilution; Thermo Fisher Scientific Inc.), rabbit polyclonal antibody against TSLP (400× dilution; Thermo Fisher Scientific Inc.), rabbit polyclonal antibody against CYP27A1 (500× dilution; Thermo Fisher Scientific Inc.), or rabbit polyclonal antibody against CYP27B1 (500× dilution; Thermo Fisher Scientific Inc.) overnight at 4 °C followed by incubation with Rat or Mouse/Rabbit Probe HRP (BioTnA, Kaohsiung, Taiwan) for 30 min. After washing, chromogen development was performed by 3,3′-diaminobenzidine staining.

    Techniques: Real-time Polymerase Chain Reaction, Immunohistochemistry, Control

    ( A ) Cytokine mRNA levels in BxPC-3, PT-45 and Capan-2 cells were assessed by real time RT-PCR and normalized to β-actin mRNA levels. Values are means (±SD) normalized gene expression. ( B ) Concentration of TSLP in CM derived from BxPC-3, PT-45, and Capan-2 cells measured by ELISA. The mean level (±SD) of cytokine detected in triplicate in CM samples is indicated.

    Journal: Oncotarget

    Article Title: Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance

    doi: 10.18632/oncotarget.25997

    Figure Lengend Snippet: ( A ) Cytokine mRNA levels in BxPC-3, PT-45 and Capan-2 cells were assessed by real time RT-PCR and normalized to β-actin mRNA levels. Values are means (±SD) normalized gene expression. ( B ) Concentration of TSLP in CM derived from BxPC-3, PT-45, and Capan-2 cells measured by ELISA. The mean level (±SD) of cytokine detected in triplicate in CM samples is indicated.

    Article Snippet: Neutralizing polyclonal antibody against TSLP (R&D Systems) or irrelevant control IgG were added to BxPC-3 cell-CM at the concentration used to neutralize TSLP biological activity (40 ng/ml) as reported above.

    Techniques: Quantitative RT-PCR, Gene Expression, Concentration Assay, Derivative Assay, Enzyme-linked Immunosorbent Assay

    Incidence of OX40L expression on normal HLA-DR + DCs obtained ( A ) in the absence (control), and in the presence of 20% BxPC-3 or Capan-2 cell-CM. Values are means ± SE. P -values obtained by One Way ANOVA followed by Bonferroni t -Test, or ( B ) in the presence of BxPC-3 cell-CM, pre-treated with a neutralizing anti-TSLP polyclonal antibody. Values are means ± SE. P -values obtained by the Paired t -Test.

    Journal: Oncotarget

    Article Title: Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance

    doi: 10.18632/oncotarget.25997

    Figure Lengend Snippet: Incidence of OX40L expression on normal HLA-DR + DCs obtained ( A ) in the absence (control), and in the presence of 20% BxPC-3 or Capan-2 cell-CM. Values are means ± SE. P -values obtained by One Way ANOVA followed by Bonferroni t -Test, or ( B ) in the presence of BxPC-3 cell-CM, pre-treated with a neutralizing anti-TSLP polyclonal antibody. Values are means ± SE. P -values obtained by the Paired t -Test.

    Article Snippet: Neutralizing polyclonal antibody against TSLP (R&D Systems) or irrelevant control IgG were added to BxPC-3 cell-CM at the concentration used to neutralize TSLP biological activity (40 ng/ml) as reported above.

    Techniques: Expressing, Control

    Basal circulating TSLP levels in PDAC patients ( n = 82) and healthy donors ( n = 41) as determined by ELISA. P -values obtained by the Mann-Whitney Rank Sum Test. Median, 10th, 25th, 75th, and 90th percentiles are presented as vertical boxes with error bars. Dots indicate outliers.

    Journal: Oncotarget

    Article Title: Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance

    doi: 10.18632/oncotarget.25997

    Figure Lengend Snippet: Basal circulating TSLP levels in PDAC patients ( n = 82) and healthy donors ( n = 41) as determined by ELISA. P -values obtained by the Mann-Whitney Rank Sum Test. Median, 10th, 25th, 75th, and 90th percentiles are presented as vertical boxes with error bars. Dots indicate outliers.

    Article Snippet: Neutralizing polyclonal antibody against TSLP (R&D Systems) or irrelevant control IgG were added to BxPC-3 cell-CM at the concentration used to neutralize TSLP biological activity (40 ng/ml) as reported above.

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Plasma levels of  TSLP  pre- and post-treatment

    Journal: Oncotarget

    Article Title: Thymic stromal lymphopoietin in human pancreatic ductal adenocarcinoma: expression and prognostic significance

    doi: 10.18632/oncotarget.25997

    Figure Lengend Snippet: Plasma levels of TSLP pre- and post-treatment

    Article Snippet: Neutralizing polyclonal antibody against TSLP (R&D Systems) or irrelevant control IgG were added to BxPC-3 cell-CM at the concentration used to neutralize TSLP biological activity (40 ng/ml) as reported above.

    Techniques: Clinical Proteomics